"A trustworthy partner for the IVD industry and R&D labs seeking premium immunoassay components."Henrik Christensen, CEO and owner of Kem-En-Tec Diagnostics

Ready-to-use pNPP Substrates


Kem-En-Tec Diagnostics pNPP substrates are liquid buffer solutions

 

Kem-En-Tec Diagnostics pNPP (p-NitroPhenyl Phosphate) substrates are liquid buffer solutions containing stabilized pNPP used for measuring Alkaline Phosphatase activity. Current methods for making pNPP-solutions involve dissolving the pNPP powder in proper buffers or diluting pNPP concentrates. The stability of these solutions is normally one day. By contrast, our ready-to-use mono-component substrates are stable up to 36 months from manufacture date when stored at 2-8°C, without loss of activity (for details see below).

  • Free of harmful organic solventspNPP - landing page Figure2
  • Aqueous based
  • Ready-to-use liquid
  • Highly active and stable formulations
  • Negligible batch-to-batch variations

 

pNPP Kem-En-Tec Diagnostics most sensitive pNPP substrate

pNPP ONE Kem-En-Tec Diagnostics most robust pNPP substrate,- recommended in hot climate countries

No harmful components

Kem-En-Tec Diagnostics pNPP substrates are aqueous based and contain NO harmful organic solvent. The non-hazardous stop solution recommended for stopping the pNPP reaction does not require harmful labeling – enabling worldwide shipping of the RTU ELISA kits.

In the presence of AP, pNPP is hydrolyzed rapidly to p-nitrophenol and inorganic phosphate. The reaction results in a water-soluble yellow end product (p-nitrophenol) that absorbs light at 405 nm. The reaction can be stopped by adding an equal amount of 0,1 M sodium hydroxide (NaOH) solution and the absorbance can be read at 405 nm. The intensity of the color is a measure of the concentration of the reactant. 

Stability data and signal-to-noise ratio of Kem-En-Tec Diagnostics pNPP substrates in a 2-layer ELISA

Stability data and signal-to-noise ratio of Kem-En-Tec Diagnostics pNPP substrates in a 2-layer ELISA

 

 

Properties of Kem-En-Tec Diagnostics Ready-To-Use pNPP substrates
  Ideal for Development time Recommended stop solution Shelf life at 2-8°C
 pNPP High sensitivity assays 15-30 min. 0,1M NaOH 2 years
 pNPP ONE Low background assays 15-30 min. 0,1M NaOH 3 years

 Properties of Kem-En-Tec Diagnostics Ready-To-Use pNPP substrates

ECO-TEK Brand Name

The ECO-TEK brand name stands for environmentally-friendly products. These products are characterized by offering the highest level of sustainability from replacing toxic components in buffer solutions to wasting less water.