Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP) are the most commonly used enzymes in immunoassays. In order to be used in the assay, HRP and AP molecules must first be conjugated to the detection antibody or antigen. The antibodies or antigens conjugated with HRP or AP can be stored at 2-8°C either as a concentrated stock solution or diluted in an appropriate buffer to the desired assay dilution factor.
When diluted biomolecules or conjugates need to be stored for future use, these buffers should also have stabilizing properties to maintain the molecular conformation and prevent loss of activity over time, finally providing ready-to-use working dilutions. Kem-En-Tec Diagnostics offers a series of different conjugate stabilizers with a stability life time of up to 4 years.
HRP-StabilPLUS suitable for the dilution and stabilization of HRP-conjugates
AP-StabilPLUS suitable for the dilution and stabilization of AP-conjugates
UNI-StabilPLUS suitable for the dilution and stabilization of both HRP- and AP-conjugates
Antibody Enhancer also able to enhance the binding capacity of the diluted antibodies for increased sensitivity
Applications and technical characteristics of Kem-En-Tec Diagnostics’ conjugate stabilizers
|Suitable for dilution and stabilization of||Stability|
|Conjugate stabilizer||HRP-conjugates||AP- conjugates||Antibodies||Shelf life at 2-8°C||Diluted conjugate shelf life at 2-8°C|
|HRP-StabilPLUS*||yes||4 years||4 years|
|AP-StabilPLUS||yes||3 years||3 years|
|UNI-StabilPLUS||yes||yes||3 years||3 years|
|Antibody Enhancer*||yes||yes||yes||4 years||4 years|
* The Antibody Enhancer & the HRP-StabilPLUS cannot be used with antibodies directed against rabbit immunoglobulins.
The ECO-TEK brand name stands for environmentally-friendly products. These products are characterized by offering the highest level of sustainability from replacing toxic components in buffer solutions to wasting less water.