Ready-to-use TMB Substrates
Kem-En-Tec Diagnostics offers a series of Liquid TMB Substrates for ELISA
Kem-En-Tec Diagnostics offers a series of Liquid TMB Substrates (3,3´, 5, 5´- tetramethyl-benzidine) for ELISA with a stability lifetime of up to 4 years. Our TMB solutions provide ready-to-use, chromogenic substrates that yield a blue color whose absorbance is read at 620-655 nm. The reaction can be stopped with a low concentration of sulfuric acid resulting in a yellow color with an absorbance read at 450 nm and a 2-3 fold increase in sensitivity compared to the non-stopped absorbance reading. The stop-solutions recommended for each product guarantee an extended stop stability of the measured absorbance, with an OD decreases after 1 hour lower than 8%.
- Free of harmful organic solvents
- Aqueous based
- Negligible batch-to-batch variations
- Recommended stop solutions do not have hazardous acid concentrations
- Environmental sustainable – ECO-TEK labelled
- A shelf life up to 4 years
TMB EASE a low concentrated TMB products for the direct transfer from 2 mg OPD tablets to the TMB platform
TMB ONE an extremely stable and robust TMB substrate
TMB PLUS2 a cost effective improved version of Kem-En-Tec Diagnostics TMB PLUS
TMB X-tra 60% higher OD values compared to the sensitivity of TMB ONE
TMB X-tnd same sensitivity as the TMB X-tra supporting a wide dynamic range. Allows OD-readings of up to 5,8 mAbs with NO precipitation within 60 min after stop of reaction
TMB SENS for high superior sensitivity and fastest assay development
Kem-En-Tec Diagnostics TMB substrates are also available as unique color-coded products for traceable pipetting. The red indicator makes manual pipetting traceable, while in automatic analyzers it offers a convenient tracking system for reagent supply. The color disappears after stopping and does not interfere with the assay.
No harmful components
- Kem-En-Tec Diagnostics TMBs are aqueous based and contain NO harmful organic solvent, including 1-methyl-2-pyrrolidone (NMP) which was added to the REACH Restricted Substances List in May 2018. This provides maximum safety during use and minimized waste problems after use. Non-hazardous acid concentration (<0.5 M H2SO4) recommended for stopping the TMB reaction do not require harmful labeling – enabling worldwide shipping of RTU ELISA kits. The products are Environmental sustainable – ECO-TEK labelled
Adjustable to Immunoassay
Kem-En-Tec Diagnostics TMB products have corresponding diluents for achieving a specific activity level. The diluted TMB solutions maintain the same stability as the undiluted products.
|Sensitivity levels and speed of color development of the different TMB formulations shown at incubation time 1-30 minutes|
|The wide range of sensitivity levels and the fast development shown by the kinetic curves allow for earlier diagnostics and reduced assay time depending on the choice of substrate.|
TMB Substrates for Elisa
|Developing time||Recommended stop solution||Max OD drop 1 h after stop||Shelf life at 2-8°C||Shelf life at RT|
|TMB ONE||5-30 min||0.2 M H2SO4||5%||4 years||1 year|
|Prestained TMB ONE||5-30 min||0.2 M H2SO4||5%||4 years||1 year|
|TMB PLUS2||5-30 min||0.2 M H2SO4||5%||4 years||1 year|
|Prestained TMB PLUS2||5-30 min||0.2 M H2SO4||5%||4 years||1 year|
|TMB X-tra||5-30 min||0.2 M H2SO4||8%||4 years||1 year|
|Prestained TMB X-tra||5-30 min||0.2 M H2SO4||8%||4 years||1 year|
|TMB SENS||5-15 min||0.3 M H2SO4||5%||2½ years||5 months|
|Prestained TMB SENS||5-15 min||0.3 M H2SO4||5%||2½ years||5 months|
|TMB EASE – NEW||20-30 min||0.2 M H2SO4||5%||4 years||1 Year|
|TMB X-Tnd – NEW||5-30 min||0.35 M H2SO4||5%||Exp. 4 years||1 Year|
Technical characteristics of Kem-En-Tec Diagnostics TMB substrates for optimal development of ELISAs
Kem-En-Tec Diagnostics TMB substrates feature a long shelf life when refrigerated or at room temperature (RT) – enabling easy shipping at RT.
ECO-TEK Brand Name
The ECO-TEK brand name stands for environmentally-friendly products. These products are characterized by offering the highest level of sustainability from replacing toxic components in buffer solutions to wasting less water.