"A trustworthy partner for the IVD industry and R&D labs seeking premium immunoassay components."Henrik Christensen, CEO and owner of Kem-En-Tec Diagnostics

Ready-to-use TMB Substrates


Kem-En-Tec Diagnostics offers a series of Liquid TMB Substrates for ELISA

 

Kem-En-Tec Diagnostics offers a series of Liquid TMB Substrates (3,3´, 5, 5´- tetramethyl-benzidine) for ELISA with a stability lifetime of up to 4 years. Our TMB solutions provide ready-to-use, chromogenic substrates that yield a blue color whose absorbance is read at 620-655 nm. The reaction can be stopped with a low concentration of sulfuric acid resulting in a yellow color with an absorbance read at 450 nm and a 2-3 fold increase in sensitivity compared to the non-stopped absorbance reading. The stop-solutions recommended for each product guarantee an extended stop stability of the measured absorbance, with an OD decreases after 1 hour lower than 8%.

 

  • Free of harmful organic solvents
  • Aqueous based
  • Ready-to-use
  • Negligible batch-to-batch variations
  • Recommended stop solutions do not have hazardous acid concentrations
  • Environmental sustainable – ECO-TEK labelled
  • A shelf life up to 4 years

TMB EASE a low concentrated TMB products for the direct transfer from 2 mg OPD tablets to the TMB platform

TMB ONE an extremely stable and robust TMB substrate

TMB PLUS2 a cost effective improved version of Kem-En-Tec Diagnostics TMB PLUS

TMB X-tra 60% higher OD values compared to the sensitivity of TMB ONE

TMB X-tnd same sensitivity as the TMB X-tra supporting a wide dynamic range. Allows OD-readings of up to 5,8 mAbs with NO precipitation within 60 min after stop of reaction

TMB SENS for high superior sensitivity and fastest assay development

 

Prestained versions

Kem-En-Tec Diagnostics TMB substrates are also available as unique color-coded products for traceable pipetting. The red indicator makes manual pipetting traceable, while in automatic analyzers it offers a convenient tracking system for reagent supply. The color disappears after stopping and does not interfere with the assay.

 

No harmful components

 

  • Kem-En-Tec Diagnostics TMBs are aqueous based and contain NO harmful organic solvent. This provides maximum safety during use and minimized waste problems after use. Non-hazardous acid concentration (<0.5 M H2SO4) recommended for stopping the TMB reaction do not require harmful labeling – enabling worldwide shipping of RTU ELISA kits. The products are Environmental sustainable – ECO-TEK labelled

 

Adjustable to Immunoassay

Kem-En-Tec Diagnostics TMB products have corresponding diluents for achieving a specific activity level. The diluted TMB solutions maintain the same stability as the undiluted products.

 immunoassay

Sensitivity levels and speed of color development of the different TMB formulations shown at incubation time 1-30 minutes

The wide range of sensitivity levels and the fast development shown by the kinetic curves allow for earlier diagnostics and reduced assay time depending on the choice of substrate.

 

 TMB Substrates

for Elisa

 Development

 Stability

Developing time  Recommended stop solution  Max OD drop  1 h after stop Shelf life at 2-8°C   Shelf life at RT
TMB ONE 5-30 min 0.2 M H2SO4 5% 4 years 1 year
Prestained TMB ONE 5-30 min 0.2 M H2SO4 5% 4 years 1 year
TMB PLUS2 5-30 min 0.2 M H2SO4 5% 4 years 1 year
Prestained TMB PLUS2 5-30 min 0.2 M H2SO4 5% 4 years 1 year
TMB X-tra 5-30 min 0.2 M H2SO4 8% 4 years 1 year
Prestained TMB X-tra 5-30 min 0.2 M H2SO4 8% 4 years 1 year
TMB SENS 5-15 min 0.3 M H2SO4 5% 2½ years 5 months
Prestained TMB SENS 5-15 min 0.3 M H2SO4 5% 2½ years 5 months
TMB EASE – NEW 20-30 min 0.2 M H2SO4 5% 4 years 1 Year
TMB X-Tnd – NEW 5-30 min 0.35 M H2SO4 5% Exp. 4 years 1 Year

 

 

Technical characteristics of Kem-En-Tec Diagnostics TMB substrates for optimal development of ELISAs

Kem-En-Tec Diagnostics TMB substrates feature a long shelf life when refrigerated or at room temperature (RT) – enabling easy shipping at RT.

 

ECO-TEK Brand Name

The ECO-TEK brand name stands for environmentally-friendly products. These products are characterized by offering the highest level of sustainability from replacing toxic components in buffer solutions to wasting less water.

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